Light Sheet Microscope

源自EMBL实验室的原创Lightsheet技术

MuVi-SPIM

IMAGING LIFE IN THE FAST LANE

Latest sCMOS camera technology and optimized combinations of objective lenses enable you to obtain the best from your sample: data you always wanted but could never get.

Intrinsic 3D optical sectioning
Large field of view
Close-to-confocal resolution
Very high imaging speed
Highest sensitivity and minimal noise

360° ILLUMINATION AND DETECTIONThe four objective lenses allow four combinations of illumination and detection. This provides four orthogonal views of the specimen without the need for sample rotation， enabling:

Multiple view imaging of large samples at unprecedented speed
Long-term stability
Time series over several days
Robust image registration
Efficient data fusion and visualization

Laser	Laser combiner with six laser positions Available laser lines 445， 488， 515， 532， 561， 594， 642， and 685 nm with 50 mW each Fast modulation and high extinction
Illumination optics	Two identical illumination arms Chromatic correction from 440 to 660 nm Light-sheet generation by beam scanning Variable light-sheet thickness (optional) Water-dipping objective lenses (see below)
Detection optics	Two identical detection arms Water-dipping objective lenses (see below) Fast filter wheels with 10 positions and 50 ms between adjacent positions Filters adapted to the selected laser lines Variable magnification (optional) High-speed sCMOS cameras Hamamatsu Orca Flash 4.0 with 2048 x 2048 pixels of 6.5 μm x 6.5 μm size
Objective lens mounting unit	High precision mounting unit Fast exchange of the unit with high precision Mounting chamber with temperature control (see below) 2 x Nikon CFI Plan Fluor 10XW 0.3 NA water immersion for illumination 2 x Nikon CFI Apo LWD 25XW 1.1 NA water immersion for detection Other objective lenses on request
Sample chamber and stage	Water-sealed inert PEEK plastic chamber， autoclavable and biocompatible Sample supported from below for improved stability Easy access from above for sample mounting， injections， etc. Fast and precise temperature control， range 15-40°C High-precision XYZ and fast rotation stage XYZ piezo crawler stage with 100 nm resolution and 8.5 mm (X) and 1.2 mm (YZ) travel range for fast sample positioning and stack acquisition Custom-made rotation stage with > 360 degrees/second speed
Electronics， Computer， Software	All electronic components included in the microscope housing Embedded microscope software with open communication interface Open GUI control for interactive control and microscope automation Computer with 64 GB RAM， Intel Dual Six Core CPU High-speed RAID controller for data streaming 8 x 4 TB local storage in RAID 0 One 40 inch monitor

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